Mario Brameshuber

Senior Scientist Dipl.-Ing. Dr.techn. Mario Brameshuber

Phone: +43 1 58801 134896 Call Mario Brameshuber

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Senior Scientist Dipl.-Ing. Dr.techn.

Phone: +43 1 58801 134896

Email: brameshuber@iap.tuwien.ac.at

Twitter: @MBrameshuber, opens an external URL in a new window

Biography

Mario Brameshuber obtained his M.S. (Dipl.Ing.) in Biophysics at Johannes Kepler University of Linz where he developed a single molecule bleaching technique termed “TOCCSL” for the detection and quantification of molecular aggregates diffusing in the plasma membrane. He completed his Ph.D. study at Johannes Kepler University of Linz in the group of Gerhard Schütz where he demonstrated the existence of stable associates of GPI-anchored proteins in the membrane of living CHO cells. After graduation Mario Brameshuber continued postdoctoral work in Linz and then moved to Stanford with a Wilhelm Macke scholarship. There he joined the group of Mark M. Davis and did postdoctoral work on addressing molecular events during T-cell activation by using single molecule techniques. Mario Brameshuber is currently university assistant at the TU Wien, group of Gerhard Schütz, and lecturer at the Vienna Medical University.

Research

His research is focusing on general features of the structure of the cellular plasma membrane by applying single molecule fluorescence techniques. His study on the homo-association of mGFP-GPI revealed the existence of structures reminiscent of highly controversially debated lipid rafts. In collaboration with experts in the field of heat shock and receptor signaling he applies the single molecule bleaching technique TOCCSL on plasma membrane targeted proteins and lipids to study their association during cellular events and under various physiological conditions. Mario Brameshuber is also studying the plasma membrane distribution and structural concepts of the T cell receptor complex in quiescent and activated murine T cells by applying TOCCSL, single molecule tracking, super-resolution techniques like STORM and PALM, and FRET. He further develops these approaches for the demand on highly sophisticated live cell techniques operating at a molecular level in order to address specific questions within his research.

Publikationen

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Teaching Experience

Lecturer and trainer for exercises to Molecular Biophysics
TU Wien
Lecturer “Molecular Biology of the Cell” and “Biomembranes”
TU Wien
Lecturer “Biophysik VO”, Post-Graduate Program of Medical Physics
Medical University of Vienna
Lecturer and trainer for exercises to Biophysics I and Biophysics II
University of Linz (JKU)
Lecturer at the Linz Winterschool Practical Course (JKU)
Course instructor at the Practical Course “Characterization of Bio - Nanostructures” (JKU)
Course instructor and lecturer at the Practical Course in Single Dye Tracing (JKU)

Education

PhD, June 2009: Doctoral Studies of Technical Sciences, PhD study (University of Linz). Dissertation in the Single Dye Tracing group of Prof. Gerhard Schütz, Institute for Biophysics:Characterization of nano structures in the live cell plasma membrane utilizing advanced single molecule fluorescence techniques’, Graduation with highest distinction
Dipl.Ing., July 2004: Diploma Studies of Technical Physics, Biophysics (University of Linz). Diploma Thesis at the Institute for Biophysics under the supervision of Prof. Gerhard Schütz: ‘TOCCSL – Eine neue Methode zur Detektion von nanoskopischen Molekülclustern (translation: A new method for detecting nanoscopic molecule clusters) Graduation with highest distinction
July 1998: Technical High School, department of mechanical engineering (Salzburg)engineer-project: ‘Construction of a new kind of 4-stroke-engine

Additional Training

Summerschool "NanoBio: The live cell from nano to micro: mind the gap!", Cargese, Corsica, France
12th Picoquant single molecule workshop, Berlin, Germany

Honors and Awards

Loschmidt price 2010 by the Chemisch-physikalische Gesellschaft, University of Vienna
PhD-thesis price 2010 by the Austrian Association of Molecular Life Sciences and Biotechnology
Wilhelm Macke Scholarship for short-term research visit in Stanford
Prize for the best Poster awarded at the 1st Annual Meeting of the Austrian Association of Molecular Life Sciences and Biotechnology (ÖGMBT)
Wilhelm Macke Award (Wilhelm Macke Scholarship Foundation) for best diploma theses in 2003/2004
Wilhelm Macke Prize (Wilhelm Macke Scholarship Foundation)

"GPI-anchored proteins do not reside in ordered domains in the live cell plasma membrane."

E. Sevcsik, M. Brameshuber, M. Folser, J. Weghuber, A. Honigmann and G. J. Schutz (2015).

Nat Commun 6: 6969.

"IgGs are made for walking on bacterial and viral surfaces."

Preiner, J., N. Kodera, J. Tang, A. Ebner, M. Brameshuber, D. Blaas, N. Gelbmann, H. J. Gruber, T. Ando and P. Hinterdorfer (2014).

Nat Commun 5: 4394.

"Single Molecule Analysis Reveals Coexistence of Stable Serotonin Transporter Monomers and Oligomers in the Live Cell Plasma Membrane."

Anderluh, A., E. Klotzsch, A. W. Reismann, M. Brameshuber, O. Kudlacek, A. H. Newman, H. H. Sitte and G. J. Schutz (2014).

J Biol Chem. 289(7): 4387-94

"A single-molecule approach to explore binding, uptake and transport of cancer cell targeting nanotubes."

Lamprecht, C., B. Plochberger, V. Ruprecht, S. Wieser, C. Rankl, E. Heister, B. Unterauer, M. Brameshuber, J. Danzberger, P. Lukanov, E. Flahaut, G. Schutz, P. Hinterdorfer and A. Ebner (2014).

Nanotechnology 25(12): 125704.

"Plasma membranes as heat stress sensors: from lipid-controlled molecular switches to therapeutic applications."

Torok, Z., T. Crul, B. Maresca, G. J. Schutz, F. Viana, L. Dindia, S. Piotto, M. Brameshuber, G. Balogh, M. Peter, A. Porta, A. Trapani, I. Gombos, A. Glatz, B. Gungor, B. Peksel, L. Vigh, Jr., B. Csoboz, I. Horvath, M. M. Vijayan, P. L. Hooper, J. L. Harwood and L. Vigh (2014).

Biochim Biophys Acta 1838(6): 1594-1618.

"Determination of binding curves via protein micropatterning in vitro and in living cells."

Sunzenauer, S., V. Zojer, Brameshuber, A. Trols, J. Weghuber, H. Stockinger and G. J. Schutz (2013).

Cytometry A 83(9): 847-854.

"A Single Peptide-Major Histocompatibility Complex Ligand Triggers Digital Cytokine Secretion in CD4+ T Cells."

Huang, J., M. Brameshuber, X. Zeng, J. Xie, Q.-j. Li, Y.-h. Chien, S. Valitutti and Mark M. Davis (2013).

Immunity 39(5): 846-857.

"Analysis of Protein-Protein Interactions in Live Cells - The Micro-Patterning Approach."

Lanzerstorfer, P., D. Borgmann, A. Steininger, S. Schaller, M. Brameshuber, S. Sunzenauer, G. J. Schuetz, O. Hoeglinger, S. Winkler and J. Weghuber (2013).

iConcept Press.

"Mapping the intracellular distribution of carbon nanotubes after targeted delivery to carcinoma cells using confocal Raman imaging as a label-free technique."

Lamprecht, C., N. Gierlinger, E. Heister, B. Unterauer, B. Plochberger, <M. Brameshuber, P. Hinterdorfer, S. Hild and A. Ebner (2012).

J Phys Condens Matter 24(16): 164206

"Detection and quantification of biomolecular association in living cells using single-molecule microscopy."

Brameshuber, M. and G. J. Schutz (2012)

Methods Enzymol 505: 159-186.

"Cationic amphipathic peptides accumulate sialylated proteins and lipids in the plasma membrane of eukaryotic host cells."

Weghuber, J., M. C. Aichinger, M. Brameshuber, S. Wieser, V. Ruprecht, B. Plochberger, J. Madl, A. Horner, S. Reipert, K. Lohner, T. Henics and G. J. Schutz (2011).

Biochim Biophys Acta 1808(10): 2581-2590

"Membrane-lipid therapy in operation: the HSP co-inducer BGP-15 activates stress signal transduction pathways by remodeling plasma membrane rafts."

Gombos, I., T. Crul, S. Piotto, B. Gungor, Z. Torok, G. Balogh, M. Peter, J. P. Slotte, F. Campana, A. M. Pilbat, A. Hunya, N. Toth, Z. Literati-Nagy, L. Vigh, Jr., A. Glatz, M. Brameshuber, G. J. Schutz, A. Hevener, M. A. Febbraio, I. Horvath and L. Vigh (2011).

PLoS ONE 6(12): e28818.

"Imaging of mobile long-lived nanoplatforms in the live cell plasma membrane."

Brameshuber, M., J. Weghuber, V. Ruprecht, I. Gombos, I. Horvath, L. Vigh, P. Eckerstorfer, E. Kiss, H. Stockinger and G. J. Schuetz (2010)

J Biol Chem285(53): 41765-71

"TCR-peptide-MHC interactions in situ show accelerated kinetics and increased affinity."

Huppa, J. B., M. Axmann, M. A. Mortelmaier, B. F. Lillemeier, E. W. Newell, M. Brameshuber, L. O. Klein, G. J. Schutz and M. M. Davis (2010)

Nature 463(7283): 963-U143

"Temporal resolution of protein-protein interactions in the live-cell plasma membrane."

Weghuber, J., S. Sunzenauer, B. Plochberger, M. Brameshuber, T. Haselgrubler and G. J. Schutz (2010).

Anal Bioanal Chem 397(8): 3339-3347

"in-vivo detection of protein-protein interactions on micro-patterned surfaces."

Weghuber, J., S. Sunzenauer, M. Brameshuber, B. Plochberger, C. Hesch and G. J. Schutz (2010).

J Vis Exp(37).

"Detection of protein-protein interactions in the live cell plasma membrane by quantifying prey redistribution upon bait micropatterning."

Weghuber, J., M. Brameshuber, S. Sunzenauer, M. Lehner, C. Paar, T. Haselgrubler, M. Schwarzenbacher, M. Kaltenbrunner, C. Hesch, W. Paster, B. Heise, A. Sonnleitner, H. Stockinger and G. J. Schutz (2010).

Methods Enzymol 472: 133-151

"Two-color single molecule tracking combined with photobleaching for the detection of rare molecular interactions in fluid biomembranes."

Ruprecht, V., M. Brameshuber and G. J. Schutz (2010).

Soft Matter6(3): 568-581

"Cholesterol Slows down the Lateral Mobility of an Oxidized Phospholipid in a Supported Lipid Bilayer."

Plochberger, B., T. Stockner, S. Chiantia, M. Brameshuber, J. Weghuber, A. Hermetter, P. Schwille and G. J. Schutz (2010)

"Plasma membrane fluidity affects transient immobilization of oxidized phospholipids in endocytotic sites for subsequent uptake"

Langmuir26(22): 17322-17329

Rhode, S., R. Grurl, M. Brameshuber, A. Hermetter and G. J. Schutz (2009)

J Biol Chem284(4): 2258-2265

"Reply to "Uncoupling diffusion and binding in FRAP experiments"

Brameshuber, M., M. Schwarzenbacher, M. Kaltenbrunner, C. Hesch, W. Paster, J. Weghuber, B. Heise, A. Sonnleitner, H. Stockinger and G. J. Schutz (2009).

Nature Methods6(3): 183-184.

"Micropatterning for quantitative analysis of protein-protein interactions in living cells"

Schwarzenbacher, M., M. Kaltenbrunner, M. Brameshuber, C. Hesch, W. Paster, J. Weghuber, B. Heise, A. Sonnleitner, H. Stockinger and G. J. Schutz (2008)

Nat Methods 5(12): 1053-1060

"How the sum of its parts gets greater than the whole"

Brameshuber, M. and G. J. Schutz (2008)

Nat Methods 5(2): 133-134

"Thinning out clusters while conserving stoichiometry of labeling"

Moertelmaier, M., M. Brameshuber, M. Linimeier, G. J. Schutz and H. Stockinger (2005)

Applied Physics Letters 87(26)

Schwarzenbacher, M., M. Kaltenbrunner, M. Brameshuber, C. Hesch, W. Paster, J. Weghuber, B. Heise, A. Sonnleitner, H. Stockinger and G. J. Schutz (2008)

"Micropatterning of CD4 in the live cell plasma membrane reveals novel binding mechanisms to Lck"

Wiener Klinische Wochenschrift 120: 20-20

Brameshuber, M. and M. Moertelmaier (2004)

"Auf Proteinjagd in der T-Zelle"

Phys. Unserer Zeit 4: 184-190

Brameshuber, M. and G. Schütz (2012). In Vivo Tracking of Single Biomolecules: What Trajectories Tell Us

Trajectories Tell Us About the Acting Forces

Springer Berlin Heidelberg: 1-37

Brameshuber, M. and G. J. Schütz (2012).

Single Molecule Measurements in Membranes. Comprehensive Biophysics.

H. E. Editor-in-Chief: Edward. Amsterdam, Elsevier: 337-365.

Direct imaging of nanoplatforms in the live cell plasma membrane. Workshop on Biomaterials and Their Interactions with Biological and Model Membranes, Salou, Spain, October 18th-22th, 2015. M. Brameshuber.
Oxidized phospholipids inhibit the formation of mobile cholesterol-dependent plasma membrane nanoplatforms.

FEBS3+Meeting Molecules of Life, Portoroz, Slovenia, September 16th-19th, 2015.
M. Brameshuber.
Oxidized phospholipids inhibit the formation of cholesterol-dependent plasma membrane nanoplatforms.

7th ÖGMBT annual meeting, Salzburg, Austria, September 9th-11th, 2015.
M. Brameshuber, E. Sevcsik, B. Rossboth, C. Manner, H. P. Deigner, A. Hermetter and G. J. Schuetz.
Techniques for direct imaging of nanoplatforms in the live cell plasma membrane.

65th Annual Meeting of the Austrian Physical Society, Vienna, Austria, September 1st-4th, 2015.
M. Brameshuber and G. J. Schuetz.
Techniques for direct imaging of mobile nanodomains in the live cell plasma membrane.

ICREA conference: Visualizing signaling nanoplatforms at a higher spatiotemporal resolution, Barcelona, Spain, May 29th-31st, 2013.

Brameshuber, M., C. Manner, J. Weghuber, H. Stockinger and G. J. Schuetz.
Imaging of mobile stable nanoplatforms in the live cell plasma membrane.

LipidomicNet Workshop: Lipid droplets and endolysosomes as dynamic organelles of lipid deposition and release, Vienna, Austria, July 9th-10th, 2012.

Brameshuber, M., J. Weghuber, V. Ruprecht, I. Gombos, I. Horvath, L. Vigh, P. Eckerstorfer, E. Kiss, H. Stockinger and G. J. Schuetz.
Techniques for imaging of nanoplatforms in the live cell plasma membrane.

Euromembrane Conference: Membrane Dynamics in Physiology and Disease, Basel, Switzerland, June 5th-8th, 2012.

Brameshuber, M., J. Weghuber, V. Ruprecht, I. Gombos, I. Horvath, L. Vigh, P. Eckerstorfer, E. Kiss, H. Stockinger and G. J. Schuetz.
Single Molecule Measurements of Membrane Protein Mobility and Interactions.

Workshop „Nanostructured Materials for Biomedical Applications Vienna-Brno-Olomouc“, Brno, Czech Republic, October 16th, 2012.

Brameshuber, M., E. Klotzsch and G. J. Schuetz.
Characterizing Plasma Membrane Structure by Thinning Out Clusters while Conserving Stoichiometry of Labeling (TOCCSL).

Invited Talk, MPI Stuttgart, Spatz Group, Stuttgart, Germany, November 28th, 2011.

Brameshuber, M.
Imaging of Mobile Stable Lipid Rafts in the live Cell Plasma Membrane and their Involvement in Cellular Signaling during Heat Shock

Biophysical Society 54th Annual Meeting, San Francisco, California, USA, February 20 - February 24, 2010.

Brameshuber, M., J. Weghuber, V. Ruprecht, I. Gombos, I. Horvath, L. Vigh, H. Stockinger and G. J. Schuetz.
Imaging of mobile stable lipid-mediated protein association in the live cell plasma membrane.

24th Annual Symposium of the Protein Society, San Diego, California, USA, August 1st - 5th, 2010.

Brameshuber, M., V. Ruprecht, J. Weghuber, I. Gombos, I. Horvath, L. Vigh, H. Stockinger and G. J. Schuetz.
Direct Observation of Plasma Membrane Rafts via Live Cell Single Molecule Microscopy.

Biophysical Society 53rd Annual Meeting, Boston, Massachusetts, USA, February 28 - March 4, 2009.

Brameshuber, M. Weghuber, V. Ruprecht, H. Stockinger and G. J. Schuetz.
Direct Observation of Plasma Membrane Rafts via Live Cell Single Molecule Microscopy.

XI. Linz Winterworkshop 2009, Linz, Austria, February 6-9, 2009.

Brameshuber, M., M. A. Moertelmaier, V. Ruprecht, H. Stockinger and G. J. Schuetz.
A Novel Method for the Stoichiometric Analysis of Molecular Aggregates in Living Cells.

Focus on Microscopy 2008, Osaka-Awaji, Japan, April 13 - 16, 2008.

Brameshuber, M., M. A. Moertelmaier, J. Weghuber, V. Ruprecht, H. Stockinger and G. J. Schuetz.
Observation of Stable GPI-GFP Clusters Diffusing in the Plasma Membrane of Living CHO cells.

Biophysical Society 51st Annual Meeting, Baltimore, Maryland, USA, March 3-7, 2007.

Brameshuber, M., M. A. Moertelmaier, J. Weghuber, V. Ruprecht, H. Stockinger and G. J. Schuetz.
TOCCSL on Living Cells.

Linz Winter School 2007, Linz, Austria, January 30 - February 1, 2007.

Brameshuber, M., M. A. Moertelmaier, J. Weghuber, V. Ruprecht, L. Muresan, H. Stockinger and G. J. Schuetz.
Observation of Stable GPI-GFP Clusters in the Plasma Membrane of Living CHO-cells.

12th International PicoQuant Workshop on "Single Molecule Spectroscopy and Ultra Sensitive Analysis in the Life Science", Berlin, Germany, September 20-22, 2006.

Brameshuber, M., J. Weghuber, V. Ruprecht, M. A. Moertelmaier, L. Muresan, H. Stockinger and G. J. Schuetz.
TOCCSL - A Novel Method for the Stoichiometric Analysis of Molecular Aggregates.

IEEE EMBS Student Club Linz, Workshop on Microscopy and Medical Image Processing, Linz, Austria, May 14-15, 2006.

Brameshuber, M., J. Weghuber, M. Linimeier, M. A. Moertelmaier, L. Muresan, H. Stockinger and G. J. Schuetz.
TOCCSL: Thinning Out Clusters while Conserving Stoichiometry of Labeling.

Nanoforum Linz 2005, Linz, Austria, May 26-27, 2005.

Brameshuber, M., M. A. Moertelmaier, M. Linimeier, H. Stockinger and G. J. Schuetz.
Observation of nanometer-sized stable structures diffusing in the plasma membrane of Jurkat T cells.

COST Meeting Utrecht, Utrecht, The Netherlands, April 14-16, 2005.

Brameshuber, M., M. A. Moertelmaier, M. Linimeier, H. Stockinger and G. J. Schuetz.

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bcookie	Is needed to store browser data (marketing/tracking).	2 years	HTTP	LinkedIn
li_oatml	Is needed to identify LinkedIn members outside of LinkedIn for advertising and analytics purposes.	1 month	HTTP	LinkedIn
BizographicsOptOut	Is needed to save privacy settings.	10 years	HTTP	LinkedIn
li_sugr	Is needed to store browser data (marketing/tracking).	3 months	HTTP	LinkedIn
UserMatchHistory	Is needed to provide advertising or retargeting (marketing/tracking).	30 days	HTTP	LinkedIn
linkedin_oauth_	Is needed to provide cross-page functionality.	session	HTTP	LinkedIn
lidc	Is needed to store performed actions on the website (marketing/tracking).	1 day	HTTP	LinkedIn
bscookie	Is needed to store performed actions on the website (marketing/tracking).	2 years	HTTP	LinkedIn
X-LI-IDC	Is needed to provide cross-page functionality (marketing/tracking).	session	HTTP	LinkedIn
AnalyticsSyncHistory	Stores the time when the user was synchronized with the "lms_analytics" cookie.	30 days	HTTP	LinkedIn
lms_ads	Is needed to identify LinkedIn members outside of LinkedIn.	30 days	HTTP	LinkedIn
lms_analytics	Is needed to identify LinkedIn members for analytics purposes.	30 days	HTTP	LinkedIn
li_fat_id	Required for indirect member identification used for conversion tracking, retargeting and analytics.	30 days	HTTP	LinkedIn
U	Is needed to identify the browser.	3 months	HTTP	LinkedIn
_guid	Is needed to identify a LinkedIn member for advertising via Google Ads.	90 days	HTTP	LinkedIn

Mario Brameshuber

Senior Scientist Dipl.-Ing. Dr.techn. Mario Brameshuber

Biography

Research

Publikationen

Teaching Experience

Education

Additional Training

Honors and Awards

Articles in Refereed Journals

Articles in Popular Journals

Contributions in Books

Scientific Talks